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KMID : 0357319760110010033
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Journal of the Korean Society for Microbiology
1976 Volume.11 No. 1 p.33 ~ p.48
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A Comparison of three Enrichment Media for Isolating Salmonella
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金英子/Kim, Yong Ja
李勝潤/朴基德/閔昌泓/Lee, Seung Yun/Park, Kee Deuk/Min, Chang Hong
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Abstract
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The practical significance of using a selective enrichment procedure for detection and enumeration of salmonella is well recognized.
There are still various selective enrichment media has been commonly used. Early years selenite broth was recommended as an enrichment media for the isolating of salmonella.
Hajna9 introduced a modified tetrathionate broth and demonstrated the greater efficiency-to compare with the- previous enrichment media.
Raj6 also described that the new medium called dulcitol selenite enrichment and has been found to be very satisfactory, especially general implication in food poisoning. Authors tried to compare these 3 enrichment media for isolating salmonella.
1. When salmonella strains were inoculated 1106 cells per tube to these 3 enrichment media, mostly similar results were obtained between selenite broth and DS broth.
In these 2 enrichment broth were showed 107/ml-108/ml cells of all tested salmonella strains.
But in the case of TT broth it was found that the growth was l0´/m1-10´/ml cells for tested strain. 2. When E. coli, Proteus, Citrobacter were inoculate 10"106 cells per tube to these 3 enrichment media. It was suggested that DS broth was showed more inhibitory action than that of selenite broth. TT broth showed high inhibition to these 3 organisms tested.
3. It was generally known that the incubation time is influenced to the frequency of salmonella detection.
For this tendency, DS broth and selenite broth were showed similar results within-24 hrs to 48hrs incubation to the test.
But DS broth showed more inhibitory action to E. coli and Proteus than that of selenite broth.
4. When 1-10 cells were inoculated(per tube) to these 3 enrichment media, DS broth was found to be more sensitive than that of selenite broth.
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